POST. MIKROBIOL.,
2000, 39, 4, 363-380 |
MECHANIZMY
INICJACJI REPLIKACJI
GENOMU BAKTERIOFAGA T4 |
Józef Nieradko, Anna M.
Ochocka
1. Wprowadzenie.
2. Matryca DNA.
3. Modyfikacje polimerazy RNA E. coli istotne w procesie inicjacji replikacji.
4. Lokalizacja origin i mechanizmy przekształcania mRNA w starter nici
wiodącej.
4.1. OriF (uvsY) i oriA.
4.2. OriE.
5. Powstawanie kompleksu replikacyjnego oraz mechanizmy syntezy starterów nici
opóźnionej.
6. Replikacja rekombinacyjna.
7. Enzymologia biosyntezy prekursorów DNA.
8. Podsumowanie |
Mechanisms initiation of Bacteriophage T4 genome
replication
Abstract: Bacteriophage
T4, one of the largest bacteriophages, encodes most, if not all, of the proteins required
for replication and recombination of its DNA. One of the most interesting aspects of T4
replication initiation is the close coupling of replication end recombination. In any
infecting chromosome, leading strand can be primed from pre-replication transcripts,
independent of primase activity, at one of several origins. Replication from T4
origins creates single-stranded genome ends which can invade homologous region of
coinfecting phage genome and thereby lead to recombinat phage. The initiation of T4 DNA
replication after wilde type infection require assembly ofthe DSC (deoxyribonucleoside
synthesising complex) and formation of dNTPs. The final proof of direct coupling and dNTP
transfer lies in functionally combining for isolated DSC and DNA replication system. |
1. Introduction.
2. DNA matrix.
3. Modification of E. coli RNA polymerase essential in initiation of origin
dependend replication.
4. Localization of particular origins and mechanisms of conversion mRNA into primer for
leading DNA strand synthesis.
4.1. OriF (uvsY) and oriA.
4.2. OriE.
5. Formation of the replication complex and mechanisms of initiation of lagging DNA strand
synthesis.
6. Recombination dependent replication.
7. Enzymology of DNA precursors biosynthesis.
8. Summary |
 pobierz dokument w formacie pdf |
 zainstaluj AcrobatReader |
|
