Browsing tag: real-time PCR

Genetyczne metody różnicowania mikroorganizmów w systemie gleba – roślina

Genetic differentiation methods of microorganisms in the soil – plant system
M. Łyszcz, A. Gałązka

1. Wstęp. 2. Elektroforeza w gradiencie czynnika denaturującego DGGE, elektroforeza w gradiencie temperatury TGGE. 3. Polimorfizm konformacji pojedynczej nici DNA SSCP. 4. Łańcuchowa reakcja polimerazy w czasie rzeczywistym (Real Time PCR). 5. Podsumowanie

Abstract: Biodiversity is a key concept in finding important features of new microorganisms. Microorganisms play an important role in the soil ecosystem and participate, among others, in such processes as the maintenance of soil structure, humification, release of organic compounds, disposal of pollutants and transformation of organic matter. The maintenance of competent state of soil microbial communities, i.e. the appropriate microorganism count, activity and diversity, is a necessary condition for the functioning of a highly complex system such as the soil. Phyllosphere bacteria have the potential to influence plant biogeography and ecosystem function through their influence on plant performance under different environmental conditions, but the drivers of variation in leaf-associated bacterial biodiversity among host plants are not well understood. Hence, undoubtedly, an important research aspect is the selection and development of indicators to evaluate microbial biodiversity of the soil and plant phyllosphere. In this publication, selected molecular methods used for the diversity assessment of microorganisms have been presented.

1. Introduction. 2. Denaturing Gradient Gel Electrophoresis DGGE, Temperature Gradient Gel Electrophoresis TGGE, 3. SSCP – single strand conformation polymorphism. 4. Real-Time Quantitative PCR. 5. Summary

Zastosowanie techniki real-time PCR w wirusologii

Application of real-time PCR in virology
S. Rynans, S. W. de Walthoffen, T. Dzieciątkowski, G. Młynarczyk

1. Wstęp. 2. Sposoby detekcji wykorzystywane w real-time PCR. 3. Zalety i wady techniki real-time PCR. 4. Zastosowanie real-time PCR w diagnostyce wirusologicznej. 5. Wykorzystanie real-time PCR w wirusologicznych badaniach naukowych. 6. Podsumowanie

Abstract: Real-time PCR is based on the revolutionary technique of polymerase chain reaction, which allows amplifying DNA more than a billion-fold. This method is used to monitor the progress of a PCR reaction in real time, and permits the detection of the number of amplicons generated during each amplification cycle. The described technique is now easy to perform, has high sensitivity and specificity, and provides the scope for automation. Within the field of microbiology, the application of real-time PCR has a significant impact upon virology. This review presents the background, advantages and limitations, as well as common applications of real-time PCR in virology, including biomedical research and laboratory diagnostics.

1. Introduction. 2. Detection formats in real-time PCR technique. 3. Advantages and limitation of the method. 4. Real-time PCR in molecular diagnostics of viral diseases. 5. Use of real-time PCR in biomedical research. 6. Summary