Zastosowanie techniki real-time PCR w wirusologii
1. Wstęp. 2. Sposoby detekcji wykorzystywane w real-time PCR. 3. Zalety i wady techniki real-time PCR. 4. Zastosowanie real-time PCR w diagnostyce wirusologicznej. 5. Wykorzystanie real-time PCR w wirusologicznych badaniach naukowych. 6. Podsumowanie
Abstract: Real-time PCR is based on the revolutionary technique of polymerase chain reaction, which allows amplifying DNA more than a billion-fold. This method is used to monitor the progress of a PCR reaction in real time, and permits the detection of the number of amplicons generated during each amplification cycle. The described technique is now easy to perform, has high sensitivity and specificity, and provides the scope for automation. Within the field of microbiology, the application of real-time PCR has a significant impact upon virology. This review presents the background, advantages and limitations, as well as common applications of real-time PCR in virology, including biomedical research and laboratory diagnostics.
1. Introduction. 2. Detection formats in real-time PCR technique. 3. Advantages and limitation of the method. 4. Real-time PCR in molecular diagnostics of viral diseases. 5. Use of real-time PCR in biomedical research. 6. Summary